A comparison between immunofluorescence staining on smears from Membrana nictitans (M3 test), immunohistopathology and routine pathology in cats with suspected feline infectious peritonitis (FIP).
Identifieur interne : 001B69 ( Main/Exploration ); précédent : 001B68; suivant : 001B70A comparison between immunofluorescence staining on smears from Membrana nictitans (M3 test), immunohistopathology and routine pathology in cats with suspected feline infectious peritonitis (FIP).
Auteurs : K. Hök [Suède]Source :
- Acta veterinaria Scandinavica [ 0044-605X ] ; 1991.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Antigènes viraux.
- anatomopathologie : Péritonite infectieuse féline.
- diagnostic : Péritonite infectieuse féline.
- immunologie : Coronavirus félin.
- microbiologie : Membrane nictitante.
- Animaux, Chats, Technique d'immunofluorescence, Valeur prédictive des tests.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Antigens, Viral.
- diagnosis : Feline Infectious Peritonitis.
- immunology : Coronavirus, Feline.
- microbiology : Nictitating Membrane.
- pathology : Feline Infectious Peritonitis.
- Animals, Cats, Fluorescent Antibody Technique, Predictive Value of Tests.
Abstract
An indirect immunofluorescence method using smears from membrana nictitans (M3 test) to diagnose feline corona virus (FCV) infection was compared with immunohistopathology (using indirect immunofluorescence assay (IFFA) performed on organs (IFO], and routine pathology (RP) in cats with suspected feline infectious peritonitis (FIP). A close correlation between the 2 immunofluorescence methods (IFO and M3) was observed. Although the M3 test requires samples from only 1 organ per animal, both the sensitivity and specificity were high (80%), when compared to IFO (using samples from an average of 5 organs per animal). In 21% of the cats with suspected FIP typical pathological lesions were found. As the M3 test is relatively easy to perform, it could reduce work-load of pathology laboratories and provide valuable data for clinical and epidemiological use.
PubMed: 1666490
Affiliations:
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Le document en format XML
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Hök</name><affiliation wicri:level="3"><nlm:affiliation>Department of Medical Microbial Ecology, Karolinska Institute, Stockholm, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Medical Microbial Ecology, Karolinska Institute, Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1991">1991</date><idno type="RBID">pubmed:1666490</idno><idno type="pmid">1666490</idno><idno type="wicri:Area/PubMed/Corpus">000D93</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000D93</idno><idno type="wicri:Area/PubMed/Curation">000D93</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000D93</idno><idno type="wicri:Area/PubMed/Checkpoint">000D72</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000D72</idno><idno type="wicri:Area/Ncbi/Merge">000090</idno><idno type="wicri:Area/Ncbi/Curation">000090</idno><idno type="wicri:Area/Ncbi/Checkpoint">000090</idno><idno type="wicri:doubleKey">0044-605X:1991:Hok K:a:comparison:between</idno><idno type="wicri:Area/Main/Merge">001B89</idno><idno type="wicri:Area/Main/Curation">001B69</idno><idno type="wicri:Area/Main/Exploration">001B69</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A comparison between immunofluorescence staining on smears from Membrana nictitans (M3 test), immunohistopathology and routine pathology in cats with suspected feline infectious peritonitis (FIP).</title><author><name sortKey="Hok, K" sort="Hok, K" uniqKey="Hok K" first="K" last="Hök">K. Hök</name><affiliation wicri:level="3"><nlm:affiliation>Department of Medical Microbial Ecology, Karolinska Institute, Stockholm, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Medical Microbial Ecology, Karolinska Institute, Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation></author></analytic><series><title level="j">Acta veterinaria Scandinavica</title><idno type="ISSN">0044-605X</idno><imprint><date when="1991" type="published">1991</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antigens, Viral (analysis)</term><term>Cats</term><term>Coronavirus, Feline (immunology)</term><term>Feline Infectious Peritonitis (diagnosis)</term><term>Feline Infectious Peritonitis (pathology)</term><term>Fluorescent Antibody Technique</term><term>Nictitating Membrane (microbiology)</term><term>Predictive Value of Tests</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Antigènes viraux (analyse)</term><term>Chats</term><term>Coronavirus félin (immunologie)</term><term>Membrane nictitante (microbiologie)</term><term>Péritonite infectieuse féline (anatomopathologie)</term><term>Péritonite infectieuse féline (diagnostic)</term><term>Technique d'immunofluorescence</term><term>Valeur prédictive des tests</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antigens, Viral</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Antigènes viraux</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Péritonite infectieuse féline</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Feline Infectious Peritonitis</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Péritonite infectieuse féline</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Coronavirus félin</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Coronavirus, Feline</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Membrane nictitante</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Nictitating Membrane</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Feline Infectious Peritonitis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cats</term><term>Fluorescent Antibody Technique</term><term>Predictive Value of Tests</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Chats</term><term>Technique d'immunofluorescence</term><term>Valeur prédictive des tests</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An indirect immunofluorescence method using smears from membrana nictitans (M3 test) to diagnose feline corona virus (FCV) infection was compared with immunohistopathology (using indirect immunofluorescence assay (IFFA) performed on organs (IFO], and routine pathology (RP) in cats with suspected feline infectious peritonitis (FIP). A close correlation between the 2 immunofluorescence methods (IFO and M3) was observed. Although the M3 test requires samples from only 1 organ per animal, both the sensitivity and specificity were high (80%), when compared to IFO (using samples from an average of 5 organs per animal). In 21% of the cats with suspected FIP typical pathological lesions were found. As the M3 test is relatively easy to perform, it could reduce work-load of pathology laboratories and provide valuable data for clinical and epidemiological use.</div></front></TEI><affiliations><list><country><li>Suède</li></country><region><li>Svealand</li></region><settlement><li>Stockholm</li></settlement></list><tree><country name="Suède"><region name="Svealand"><name sortKey="Hok, K" sort="Hok, K" uniqKey="Hok K" first="K" last="Hök">K. Hök</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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